发明名称 |
Synthetic surfaces for culturing cells in chemically defined media |
摘要 |
Synthetic surfaces capable of supporting culture of eukaryotic cells including stem cells and undifferentiated human embryonic stem cells in a chemically defined medium include a swellable (meth)acrylate layer and a polypeptide conjugated to the swellable (meth)acrylate layer. The swellable (meth)acrylate layer may be formed by polymerizing monomers in a composition that inclucles a carboxyl group-containing (meth)acrylate monomer, a cross-linking (di- or higher-functional) (meth)acrylate monomer, and a hydrophilic monomer capable of polymerizing with the carboxyl group-containing (meth)acrylate monomer and the cross-linking (meth)acrylate monomer. The swellable (meth)acrylate layer has an equilibrium water content in water of between about 5% and about 70%. The conjugated peptide may include an RGD amino acid sequence. |
申请公布号 |
EP2420564(A3) |
申请公布日期 |
2013.12.25 |
申请号 |
EP20110188775 |
申请日期 |
2009.01.30 |
申请人 |
GERON CORPORATION |
发明人 |
FADEEV, ANDREI;GEHMAN, JENNIFER;MELKOUMIAN, ZARA;WEBER, DAVID M;ZHOU, YUE |
分类号 |
C12N5/00;C08F220/28;C08F222/10;C08F226/10 |
主分类号 |
C12N5/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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