| 摘要 |
<p>The invention provides a method of converting hydrogen and carbon dioxide into methane by methanogenic microorganisms in a reaction vessel, comprising contacting the methanogenic microorganisms with in-gas comprising hydrogen and carbon dioxide. The method comprises at least one methane production phase, wherein in the at least one methane production phase the ratio of the partial pressure of hydrogen to the partial pressure of carbon dioxide inside the reaction vessel is adjusted to 5:1 or higher (parts hydrogen : parts carbon dioxide). The method of the invention may optionally comprise at least one growth phase, wherein in the at least one cell growth phase the ratio of the partial pressure of hydrogen to the partial pressure of carbon dioxide inside the reaction vessel is adjusted to a ratio different from the ratio in the at least one methane production phase. The invention also provides a bioreactor suitable for the method of the invention of converting hydrogen and carbon dioxide into methane by methanogenic microorganisms. In addition, methanogenic microorganisms are provided which are having a biomass concentration of at least 6 g biomass per litre, wherein no additional methanogenic microorganism is added after inoculation or cell retention is applied during fermentation, and also the use of methanogenic microorganisms having a biomass concentration of at least 6 g biomass per litre under continuous fermentation conditions is provided.</p> |
