Quantitative nuclease protection Assay (qNPA) and sequencing (qNPS) improvements
摘要
The present disclosure provides an improvement to quantitative Nuclease Protection Assay (qNPA) and quantitative Nuclease Protection Sequencing (qNPS) methods. The disclosed methods use nuclease protection probes (NPPs) that include 5'-end and/or 3-end flanking sequences, which provide a universal hybridization and/or amplification sequence. The disclosed methods can be used to sequence or detect target nucleic acid molecules, such as those present in fixed or insoluble samples.
申请公布号
AU2012251027(A1)
申请公布日期
2013.12.19
申请号
AU20120251027
申请日期
2012.04.26
申请人
HTG MOLECULAR DIAGNOSTICS, INC.
发明人
SELIGMANN, BRUCE;THOMPSON, DEBRAH;VASICEK, TOM;GORDON, DEBRA A.