发明名称 |
Methods and compositions for nucleic acid amplification |
摘要 |
Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample. |
申请公布号 |
AU2007336839(C1) |
申请公布日期 |
2013.12.19 |
申请号 |
AU20070336839 |
申请日期 |
2007.12.20 |
申请人 |
GEN-PROBE INCORPORATED |
发明人 |
LYAKHOV, DMITRY;CARLSON, JAMES D.;NELSON, NORMAN C.;BRENTANO, STEVEN T.;ARNOLD JR., LYLE J. |
分类号 |
C12P19/34 |
主分类号 |
C12P19/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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