PRIMERS FOR THE SIMULTANEOUS DETECTION OF F4, F5, F6, F18, F41 IN E. COLI AND MULTIPLEX PCR KITS USING FORMER PRIMERS

摘要

The present invention relates to a primer set for detecting an E. coli fimbrial gene and a multiplexe polymerase chain reaction kit using the same, and more particularly, to: (1) a primer set for detecting E. coli fimbrial gene F4, comprising a forward primer, which has sequence number 1, and a reverse primer, which has sequence number 2; (2) a primer set for detecting E. coli fimbrial gene F5, comprising a forward primer, which has sequence number 3, and a reverse primer, which has sequence number 4; (3) a primer set for detecting E. coli fimbrial gene F6, comprising a forward primer, which has sequence number 5, and a reverse primer, which has sequence number 6; (4) a primer set for detecting E. coli fimbrial gene F18, comprising a forward primer, which has sequence number 7, and a reverse primer, which has sequence number 8; and (5) a primer set for detecting E. coli fimbrial gene F41, comprising a forward primer, which has sequence number 9, and a reverse primer, which has sequence number 10, and a multiplexe polymerase chain reaction kit capable of simultaneously detecting five types of E. coli, which are F4, F5, F6, F18, and F41, using at least one primer set selected from the primer sets of (1) to (5).