摘要 |
<p>Obtaining a derivative of mono-N-deacetylated chito-oligosaccharide (I) comprises (a) performing in vitro hydrolysis of N-acetyl group of N-acetylglucosamine second unit of non-reducing side of a derivative of chito-oligosaccharides (II) with a preparation of recombinant N-deacetylased chitin oligosaccharide of Vibrio cholerae to obtain a mono-N-deacetylated chito-oligosaccharide derivative (III), and eliminating non-reducing saccharide unit of (III) with beta -N-acetyl-hexosaminidase to obtain (I). Obtaining a derivative of mono-N-deacetylated chito-oligosaccharide of formula (I) comprises (a) performing in vitro hydrolysis of N-acetyl group of N-acetylglucosamine second unit of non-reducing side of a derivative of chito-oligosaccharides of formula (II) with a preparation of recombinant N-deacetylased chitin oligosaccharide of Vibrio cholerae to obtain a mono-N-deacetylated chito-oligosaccharide derivative of formula (III), and eliminating non-reducing saccharide unit of (III) with beta -N-acetyl-hexosaminidase to obtain (I). R1 : H, 1-6C-alkyl, fucosyl, methylfucosyl, sulfofucosyl, acetylfucosyl, arabinosyl, SO 3H, SO 3Li, SO 3Na, SO 3K or SO 3N(1-8C-alkyl); and n : 0-5. Independent claims are included for: (1) obtaining a derivative of mono-N-deacetylated chito-oligosaccharide of formula (IV) comprising the steps (a) and (b) as above per se, and (c) performing N-acylation step of (I) with lipid radical to obtain (IV); and (2) obtaining a derivative of mono-N-deacetylated chito-oligosaccharide of formula (V) comprising the steps (a) as above per se, and (b1) performing N-acylation step of (III) with lipid radical to obtain (V). R2 : lipid acyl radical. [Image] [Image].</p> |