| 摘要 |
PURPOSE: A novel F3'H gene relating to biosynthesis of cyanidin isolated from Dendranthema grandiforum is provided to develop novel Dendranthema grandiforum species. CONSTITUTION: A polypeptide having flavonoid 3'-hydroxylase(F3'H) activity, mutant thereof, or active fragment thereof includes: a polypeptide encoded by cDNA of sequence numbers 1, and 10-14; a polypeptide encoded by a polynucleotide hybridized with cDNA of sequence numbers 10-14 under a severe condition; a polypeptide encoded by gDNA of sequence numbers 2, and 15-19; a polypeptide encoded by a polynucleotide hybrized with the gDNA under a severe condition; or a polypeptide having an amino acid sequence of sequence numbers 3 and 20-24. The polypeptide, mutant thereof, or active fragment thereof is derived from Dendranthema grandiforum. |
