发明名称 |
A method and an apparatus for localization of single dye molecules in the fluorescent microscopy |
摘要 |
The invention concerns a method and a corresponding apparatus for obtaining a sub-resolution spatial information of a sample labeled with at least one type fluorescent label, said sub-resolution spatial information comprising localization information about the positions of fluorescent molecules of the at least one type fluorescent label in at least one spatial direction. The method comprises: - acquiring localization image data by employing fluorescence localization microscopy, wherein said localization image data comprises a series of images obtained by - illuminating a region of interest of the sample with illumination light having intensity in the range of approximately 1 kW/cm |
申请公布号 |
AU2009226687(B2) |
申请公布日期 |
2013.12.12 |
申请号 |
AU20090226687 |
申请日期 |
2009.03.13 |
申请人 |
RUPRECHT-KARLS-UNIVERSITAT HEIDELBERG |
发明人 |
LEMMER, PAUL;BADDELEY, DAVID;CREMER, CRISTOPH;EIPEL, HEINZ |
分类号 |
G01N21/64;G02B21/00 |
主分类号 |
G01N21/64 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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