| 摘要 |
Methods and compositions for controlling gene expression are disclosed. In accordance with the present invention, compositions and methods are provided for modulating the expression of a gene of interest. Specifically, nucleic acid molecules encoding a polypeptide comprising a ONA binding domain and a looping factor are provided. The DNA binding domain and the looping factor may be linked through a covalent bond or an amino acid linker. In a particular embodiment, the DNA binding domain comprises a zinc finger polypeptide or a transcription activator-like effector polypeptide. The DNA binding domain may be designed to specifically bind a target sequence in the promoter of a gene of interest to modulate its expression. |
