发明名称 |
MODIFIED BASE DETECTION WITH NANOPORE SEQUENCING |
摘要 |
Methods, compositions, and systems are provided for characterization of modified nucleic acids. Nanopore sequencing is performed using a processive enzyme to control the rate of translation of a single strand of a nucleic acid through a nanopore. The presence and identity of a modified base can be determined by monitoring the kinetics of translation through the pore even though the events that lead to the kinetic changes are separated in time and space from the translation of the modified base or it's compliment through the nanopore. The invention also comprises the use of hemi-genomic DNA in nanopore sequencing. |
申请公布号 |
US2013327644(A1) |
申请公布日期 |
2013.12.12 |
申请号 |
US201313914361 |
申请日期 |
2013.06.10 |
申请人 |
PACIFIC BIOSCIENCES OF CALIFORNIA, INC. |
发明人 |
TURNER STEPHEN;KORLACH JONAS |
分类号 |
G01N27/447 |
主分类号 |
G01N27/447 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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