SCREENING METHOD, PROTEIN INSTABILITY AND/OR STABILITY INDUCERS, AND PROTEIN ACTIVITY ASSESMENT

摘要

Provided is a screening method with which it is possible to determine the cause of a decrease or an increase in the amount of a target protein that is expressed by a cell. One or a plurality of embodiments include: cultivation of a culture including an assay cell brought into contact with a test substance, and measurement of the relative amount of the target protein (A) in relation to an internal standard; cultivation of a culture in which an assay cell is not contacted with a test substance, and measurement of the relative amount (B) in relation to an internal standard; comparison of the relative amount (A) and the relative amount (B); and, based on the comparison, selection of a candidate substance that induces instability and/or stability in the target protein. The assay cell is a cell that is able to express the mRNA of the target protein and the mRNA of the internal standard protein under identical regulation of expression, or a cell having a means for expressing the mRNA of the target protein and the mRNA of the internal standard protein under conditions where the gene expression control is identical.