摘要 |
<p>Measurements having reproducibility, in which fluctuations in intensities of electric field enhancing fields that occur at each measurement are suppressed, are realized simply and at low cost, in fluorescence detection that utilizes surface plasmon. The intensity of scattered light (Ls), which is substantially proportionate to the intensity of an electric field enhancing field (Ew) generated on a metal film (12), is employed, to normalize and correct the intensity of fluorescence (Lf) emitted by fluorescent labels (F) with respect to the intensity of the electric field enhancing field (Ew).</p> |