发明名称 |
Extract of E. coli cells having mutation in ribosomal protein S12, and method for producing protein in cell-free system using the extract |
摘要 |
It is intended to provide an extract, kit and a process for synthesizing a protein in a cell-free system. The extract is characterized by being prepared from mutant Escherichia coli cells having a mutation in the ribosomal protein S12 gene. In a preferred embodiment, the mutation is such a mutation as conferring a resistance or dependence to streptomycin on E. coli whereby the reading efficiency of mRNA codon on ribosomes can be improved and thus the protein productivity can be elevated. |
申请公布号 |
US8603774(B2) |
申请公布日期 |
2013.12.10 |
申请号 |
US20050137395 |
申请日期 |
2005.05.26 |
申请人 |
CHUMPOLKULWONG NAMTIP;HORI CHIE;YOKOYAMA SHIGEYUKI;SHIROUZU MIKAKO;KIGAWA TAKANORI;OCHI KOZO;HOSAKA TAKESHI;NATIONAL FOOD RESEARCH INSTITUTE;RIKEN |
发明人 |
CHUMPOLKULWONG NAMTIP;HORI CHIE;YOKOYAMA SHIGEYUKI;SHIROUZU MIKAKO;KIGAWA TAKANORI;OCHI KOZO;HOSAKA TAKESHI |
分类号 |
C12N15/09;C12P21/02 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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