摘要 |
PROBLEM TO BE SOLVED: To figure out more exactly the true spectral intensity on the basis of a measured spectrum.SOLUTION: An information processing apparatus comprises: an assaying unit that statistically assays each frequency band by using, as objects of comparison, simple-stained data obtained by fluorescence measuring of particles simply stained with a staining substance having prescribed fluorescence characteristics and non-stained data obtained by fluorescence measuring of unstained particles; a masking unit that sets, for each frequency band, in the absence of any significant difference between the simple-stained data and the non-stained data, the simple-stained data to 0 or a prescribed value; and an estimating unit that estimates, on the basis of the assumption that double-stained data obtained by fluorescence measuring of particles stained with a plurality of staining substances is expressed by a linear combination of base vectors representing the distribution of pieces of simple-stained data each corresponding to each staining substance, the combination coefficient of the pertinent linear combination. |