NEW METHOD FOR THE ISOLATION OF THE PROTEINS BOUND TO ANY KIND OF INTERESTING NUCLEIC ACID SEQUENCE

摘要

The invention is to supply a novel method for isolation of the proteins bound to any kind of interesting nucleic acid sequence (Chromatin of Interest (Col)),wherein -in a first step a Triplex Forming Tags cassette(TFT cassette) is introduced in said nucleic acid sequence of a living cell and said living cells are grown; -in a second step cells obtained in step 1 are collected and mixed with at least one molecular probe (the TFO probe) specific of at least one of the introduced TFT sequences of the TFT cassette in conditions that permit the formation of nucleic acid triplex; -in a third step the nucleic acid triplex formed in second step are isolated and bound proteins are analyzed, characterized in that the TFT cassette comprises at least 2 contiguous TFT sequences.