摘要 |
PROBLEM TO BE SOLVED: To develop technology for separately refining a low molecular useful component with high purity, such as trigonelline, from coffee, black tea, tea, cocoa, or the like by a simple method.SOLUTION: A stationary phase and a mobile phase are created in extraction liquid obtained from raw materials by using solution of a constitution made from butanol/water/acetic acid to separately refine high-speed counter-current chromatography. |