发明名称 |
VIRUS CAPABLE OF EXPRESSING -GALACTOSE EPITOPE, AND METHOD FOR PRODUCTION OF VACCINE |
摘要 |
<p>A method for producing an α-Gal-expressing virus having enhanced immune response to viruses, without requiring the use of any enzyme; an influenza virus vaccine having a high effect (antigenicity), which is produced using an α-Gal-expressing virus produced by the method; and others. Specifically disclosed are: a method for producing an α-galactose epitope (Galα1-3Galβ1-4GlcNAc -R: α-Gal hereinafter)-expressing virus, which comprises the steps of:
(1) introducing an α1,3-galactosyltransferase gene in an expressible condition into a cell line that does not express α-Gal to obtain a cell line capable of expressing α-Gal;
(2) inoculating a virus into the cell line capable of expressing α-Gal to obtain a cell line infected with a virus; and
(3) culturing the cell line infected with the virus to obtain a virus expressing α-Gal from the culture medium.</p> |
申请公布号 |
EP2484757(A9) |
申请公布日期 |
2013.12.04 |
申请号 |
EP20100820641 |
申请日期 |
2010.09.30 |
申请人 |
NATIONAL UNIVERSITY CORPORATION OBIHIRO UNIVERSITY OF AGRICULTURE AND VETERINARY MEDICINE;INCORPORATED ADMINISTRATIVE AGENCY NATIONAL AGRICULTURE AND FOOD RESEARCH ORGANIZATION |
发明人 |
OGAWA, HARUKO;IMAI, KUNITOSHI;TAGAMI, TAKAHIRO |
分类号 |
C12N7/00;A61K39/145;A61K39/165;A61K39/17;A61K39/20;A61K39/21;A61K39/255;A61K39/285;A61P31/12;A61P31/16;C12N7/04;C12N9/10;C12N15/09 |
主分类号 |
C12N7/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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