发明名称 |
METHOD FOR IDENTIFYING POLYMORPHISM OF NUCLEIC ACID MOLECULES |
摘要 |
The present invention provides a method for identifying polymorphism of nucleic acids in a sample solution in which the concentration or number density of the observed nucleic acids is lower than that of conventional photometric analysis technologies. The method includes: a step of preparing a sample solution comprising a first nucleic acid probe, which specifically hybridizes with a single-stranded nucleic acid molecule including a first type of base sequence in a polymorphic sequence, and a target nucleic acid molecule; a step of forming a hybrid of the nucleic acid molecules in the sample solution; a step of calculating a number of molecules of the hybrid including the first nucleic acid probe in the sample solution by the scanning molecule counting method; and a step of identifying polymorphism of the target nucleic acid molecule based on the calculating result. The sample solution includes an oligonucleotide having a base sequence complementary to a base sequence different from the first type of base sequence in the polymorphic sequence. |
申请公布号 |
EP2669376(A1) |
申请公布日期 |
2013.12.04 |
申请号 |
EP20120739756 |
申请日期 |
2012.01.26 |
申请人 |
OLYMPUS CORPORATION |
发明人 |
NISHIKAWA, Kazutaka;NAKATA, Hidetaka |
分类号 |
C12N15/09;C12Q1/68;G01N21/64;G01N21/78 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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