| 摘要 |
Provided is a method of selecting a polypeptide sequence which imparts a target substance binding-ability to a protein of interest, said method comprises repeating the following steps (1) to (3);
(1) expressing a library of fusion proteins, each of which is composed of a random polypeptide and a protein of interest or part thereof, from a nucleic acid construct comprising a first nucleotide sequence encoding the random polypeptide and a second nucleotide sequence which is linked to the first nucleotide sequence in flame and encodes the protein of interest or part thereof,
(2) contacting the library of fusion proteins with a target substance,
(3) selecting a fusion protein which comprises a polypeptide sequence that binds to the target substance, and amplifying a nucleic acid construct which encodes the selected fusion protein. Further, provided is a peptide including the following amino acid sequence. Tyr-Xaa 0 -Xaa 1 -Tyr-Tyr-Xaa 2 -Xaa 3 -Tyr-Xaa 4 -Xaa 5 -Xaa 6 -Xaa 7 -Xaa 8 -Xaa 9 -Xaa 10 -Xaa 11 (SEQ ID NO: Y: wherein Xaa 0 , Xaa 1 , Xaa 2 , Xaa 3 , Xaa 4 , Xaa 5 , Xaa 6 , Xaa 7 , Xaa 8 , Xaa 9 , Xaa 10 and Xaa 11 represent any amino acid) or Tyr-Asn-Asp-Tyr-Tyr-Tyr-Tyr-Cys-Tyr-Arg-Asp-Tyr-Asp. |
