| 摘要 |
PURPOSE: A Corynebacterium glutamicum mutant strain is provided to supply enough L-threonine without the suppression of L-isoleucine feedback, thereby producing L-isoleucine with high efficiency and high yield. CONSTITUTION: A Corynebacterium glutamicum mutant strain (KCCM11248P) produces L-isoleucine. The mutant strain has resistance to L-isoleucine, L-threonine, and derivatives thereof. The L-isoleucine derivative is 4-thiaisoleucine (thiaile) or isoleucine-hydroxamate (ileHx). The L-threonine derivative is alpha-amino-beta-hydroxynorvaline (AHV). A method for producing L-isoleucine comprises the steps of: culturing the mutant strain (KCCM11248P); and collecting L-isoleucine from a culture of the mutant strain. [Reference numerals] (AA) L-leucine; (BB) Aminotransferase (ilvE); (CC) Ketoisocaproic acid; (DD) Isopropylmalic acid dehydrogenase (leuB); (EE) 3-isopropylmalic acid; (FF) Isopropylmalic acid dehydratase (leuD); (GG) 2-isopropylmalic acid; (HH) Isopropylmalic acid synthase (leuA); (II) L-valine; (JJ) Ketoisovaleric acid; (KK) Dihydroxyisovaleric acid; (LL) Acetolactic acid; (MM,NN,OO) Pyruvic acid; (PP) Acetohydroxyacid synthase (ilvBN); (QQ) Acetohydroxyacid isomer reductase (ilvC); (RR) Dihydroxy acid dehydratase (ilvD); (SS) Aminotransferase (ilvE); (TT) L-threonine; (UU) Threonine dehydratase (ilvA); (VV) 2-ketobutyric acid; (WW) Acetohydroxy butyric acid; (XX) Dihydroxy methyl valeric acid; (YY) Ketomethyl valeric acid; (ZZ) L-isoleucine |
