发明名称 |
SSO7-POLYMERASE CONJUGATES WITH DECREASED NON-SPECIFIC ACTIVITY |
摘要 |
<p>Improved Sso7-polymerase conjugate proteins are provided. The present invention provides for an Ss07 polymerase conjugate protein comprising an Ss07 domain linked to a polymerase; wherein: an amino acid of the Ss07 domain corresponding to K28 of SEQ 10 NO:2 is not lysine (K); and/or an amino acid of the Ss07 domain corresponding to R43 of SEQ ID NO:2 is not arginine (R), wherein the conjugate protein has a decreased non-specific amplification activity compared to an otherwise identical control conjugate protein in which the amino acid of the Ss07 domain corresponding to K28 of SEQ ID NO:2 is lysine (K) and the amino acid of the Ss07 domain corresponding to R43 of SEQ ID NO:2 is arginine (R).</p> |
申请公布号 |
SG194032(A1) |
申请公布日期 |
2013.11.29 |
申请号 |
SG20130073515 |
申请日期 |
2012.02.10 |
申请人 |
BIO-RAD LABORATORIES, INC. |
发明人 |
CHENG, MAN;WANG, YAN;SULLIVAN, JOHN |
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