发明名称 |
MEMBRANE-SEPARATION-TYPE CULTURE DEVICE, MEMBRANE-SEPARATION-TYPE CULTURE KIT, STEM CELL SEPARATION METHOD USING SAME, AND SEPARATION MEMBRANE |
摘要 |
<p>A membrane separation culture device 1, which comprises an upper structure 10 constituted with a vessel comprising a membrane 12 having, in at least a portion thereof, pores 121 that allow stem cells to permeate therethrough, and a lower structure 13 constituted 5 with a vessel that retains a fluid in which the membrane of the upper structure is immersed; a membrane separation culture kit comprising the membrane separation culture device 1 and cell migration factor(s); a method for separating stem cells, which comprises a step of dispersing test cells or test tissues on the membrane 12 of the upper structure 10, a step of filling the lower structure 13 with a medium containing the cell migration factor(s), and a step 10 of allowing the membrane 12 of the upper structure 10 to come into contact with the medium in the lower structure 13; and a separation membrane comprising a base material membrane consisting of a hydrophobic polymer and a functional layer formed by allowing one or morehydrophilic polymers selected from a vinyl pyrrolidone polymer, a polyethylene glycol polymer and a vinyl alcohol polymer to bind to the surface of the base material membrane via 15 a covalent bond, wherein the weight percentage of the hydrophilic polymer(s) constituting the functional layer is 1.5% to 35% based on the total weight of the separation membrane.</p> |
申请公布号 |
SG194029(A1) |
申请公布日期 |
2013.11.29 |
申请号 |
SG20130073473 |
申请日期 |
2012.03.30 |
申请人 |
NATIONAL CENTER FOR GERIATRICS AND GERONTOLOGY;TORAY INDUSTRIES, INC. |
发明人 |
NAKASHIMA, MISAKO;IOHARA, KOICHIRO;YAMADA, KAZUMASA;SHIMAGAKI, MASAAKI;OSABE, MASAHIRO |
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