发明名称 |
REAGENTS AND METHODS FOR IDENTIFYING ANTI-HIV COMPOUNDS |
摘要 |
<p>Single-molecule imaging techniques have been applied herein and have demonstrated the conformation states of HIV-1 Env. The introduction of small organic fluorophores at selected positions within HIV-1 Env that do not affect infectivity permits the detection of changes in inter-dye distances as a measure of conformational changes. Implementation of the smFRET-based technologies disclosed herein enable conformational screening for molecules that block, induce or trap HIV-1 Env in specific conformational states. Methods for identifying anti-HIV drugs in a smFRET-based screening, and various reagents useful for implementation of such methods, are provided.</p> |
申请公布号 |
WO2013177294(A1) |
申请公布日期 |
2013.11.28 |
申请号 |
WO2013US42249 |
申请日期 |
2013.05.22 |
申请人 |
CORNELL UNIVERSITY;YALE UNIVERSITY |
发明人 |
BLANCHARD, SCOTT C.;MOTHES, WALTHER;MUNRO, JAMES |
分类号 |
C07K14/155;C12N15/09;C12N15/33;C12N15/79;G01N33/58;G01N33/68 |
主分类号 |
C07K14/155 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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