METHODS AND COMPOSITIONS FOR RNA-DIRECTED TARGET DNA MODIFICATION AND FOR RNA-DIRECTED MODULATION OF TRANSCRIPTION
摘要
The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.
申请公布号
CA2872241(A1)
申请公布日期
2013.11.28
申请号
CA20132872241
申请日期
2013.03.15
申请人
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA;UNIVERSITY OF VIENNA;CHARPENTIER, EMMANUELLE
发明人
JINEK, MARTIN;DOUDNA CATE, JAMES HARRISON;LIM, WENDELL;QI, LEI;DOUDNA, JENNIFER A.;CHYLINSKI, KRZYSZTOF;CHARPENTIER, EMMANUELLE