| 摘要 |
PURPOSE: Armillaria gemina which produces cellulase and a use thereof for saccharification are provided to ensure excellent saccharification and to be used in various purposes such as production of low calorie foods and fermentation of food waste. CONSTITUTION: A composition for producing cellulase contains Armillaria gemina(deposit number KCCM 11186BP) which produces cellulase or a culture medium thereof as an active ingredient. A method for producing cellulase comprises a step of culturing Armillaria gemina and isolating cellulase. The culture medium of cellulase contains 10-30 g/L of rice straws, 8-10 g/L of corn steep, 2-5 g/L of yeast extract, 3-5 g/L of monobasic potassium phosphate, 3-5 g/L of potassium diphosphate, and 0.005-0.01 g/L of thiamine HCl. Cellulase is produced at 250rpm, at an infiltration rate of 0.8-1.2vvm, in pH 5, and at 30 deg. C. |
