A modified outlet end fitting and frit for reaction chromatography

摘要

A chromatography column having a fluid outlet (305) configured with two or more fluid ports (301, 302), of which are: one or more reactant ports connecting a reactant flow from a reactant source with the eluate flow, and one or more are product ports being in communication with one or more processing units to process the eluate flow. The ports may be positioned to react with a portion of the eluate flowing from restricted regions of the column to allow for different processing, specifically the central region (301) separately from its peripheral region (302). Processing units may be a detector, a fraction collector and/or another chromatography column. The column outlet may further comprise a split frit (303) for further segmentation of the eluent. Also given is the method of reaction chromatography including the introduction and reaction of a reactant flow with the eluate flow at the outlet of the column.