发明名称 |
NUCLEIC ACID CONSTRUCT FOR EXPRESSING OXIDATIVE STRESS INDICATOR AND USE THEREOF |
摘要 |
<p>The present invention provides a nucleic acid construct for expressing an oxidative stress indicator comprising: a nucleic acid sequence encoding an Nrf2 protein-derived partial protein that comprises at least an Neh2 domain sequence and substantially lacks or is functionally deficient in an Neh1 domain sequence or an Neh1-Neh3 domain sequence; a stress-inducible promoter sequence positioned upstream of the nucleic acid sequence encoding an Nrf2 protein-derived partial protein; and a nucleic acid sequence encoding a protein capable of generating a detectable signal, the nucleic acid sequence being positioned downstream of the nucleic acid sequence encoding an Nrf2 protein-derived partial protein. The present invention also provides a method for measuring oxidative stress and a method for screening for an anti-oxidative stress agent, using the nucleic acid construct.</p> |
申请公布号 |
EP2666857(A1) |
申请公布日期 |
2013.11.27 |
申请号 |
EP20120736230 |
申请日期 |
2012.01.20 |
申请人 |
RIKEN |
发明人 |
IWAWAKI, TAKAO;OIKAWA, DAISUKE |
分类号 |
C12N15/09;A01K67/027;C12N1/15;C12N1/19;C12N1/21;C12N5/10;G01N21/78;G01N33/15;G01N33/50 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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