| 摘要 |
The invention refers to a method for in-vitro analysis of biological cells and/or microorganisms characteristics selected from the group consisting of degree of differentiation, cell types, donor individuals, culture conditions, purity, lack of natural characteristic, additional characteristic in comparison to natural characteristics, comprising the steps of: (a) projecting infrared radiation on a sample of biological cells and/ or microorganisms, said radiation including a selected number of wavelengths; (b) recording the spectral characteristics after interaction with said sample; (c) deriving a Fourier transform infrared spectrum (FT-IR) from the collected spectral characteristics; (d) generating the first or multiple derivative transformation of the FT-IR spectrum suitable for analysis in a computer; (e) comparing said derivative with the derivative of a reference FT-IR spectrum determined earlier; (f) identifying deviations of said derivative from said reference derivative; (g) providing an analysis of said characteristics based on the presence of said deviations. In addition, the invention relates to an apparatus for carrying out this method as well as to the use of this method and said apparatus in the field of transplantation/graft and tissue engineering, clinical medicine, pharmaceutical industry, quality control and/or drug safety.
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