Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same
摘要
Compositions are provided, which can be used as frameworks for the creation of very stable and soluble single-chain Fv antibody fragments. These frameworks have been 5 selected for intracellular performance and are thus ideally suited for the creation of scFv antibody fragments or scFv antibody libraries for applications where stability and solubility are limiting factors for the performance of antibody fragments, such as in the reducing environment of a cell. Such frameworks can also be used to identify highly conserved residues and consensus sequences which demonstrate enhanced solubility and 10 stability.
申请公布号
AU2012211497(B2)
申请公布日期
2013.11.21
申请号
AU20120211497
申请日期
2012.08.01
申请人
ESBATECH, AN ALCON BIOMEDICAL RESEARCH UNIT LLC
发明人
TISSOT, KATHRIN;AUF DER MAUR, ADRIAN;ESCHER, DOMINIK;EWERT, STEFAN;BARBERIS, ALCIDE