TEST SYSTEM FOR DETECTION OF BLUETONGUE RNA VIRUS OF 14-TH SEROTYPE USING OT-PCR METHOD IN REAL-TIME MODE

摘要

FIELD: biotechnologies.SUBSTANCE: test system for detection of bluetongue RNA virus of the 14-th serotype by means of a polymerase chain reaction in a real-time mode includes oligonucleotide primers and a DNA probe, which are complementary to sections of variable 2-nd segment, which have the following nucleotide composition (5'-3'): 14/2/qf GCT GTC GGG TAT AGG TAG GA; 14/2/z FAM - GTG AAC GTG GGG TCA TCT TCA C - BHQ1; 14/2/qr ACG TGT CCG ATG CTG CTA TC.EFFECT: test system has increased specificity and sensitivity at reduction of the time required for performance of an operation relating to determination of a serotype of bluetongue virus.2 tbl, 1 ex