Separation system, components of a separation system and methods of making and using them

摘要

The invention is related to a method of making a monolithic chromatography column comprising the steps of adding a polymerization mixture containing a porogen to a closed container, polymerizing the mixture under pressure to form a polymer plug whereby sufficient pressure is applied to prevent voids being formed caused by vacuum due to shrinkage during polymerization mixture to a controlled elevated temperature, and washing the polymer plug to remove the porogen.