CHIMERIC PROTEIN BEING FLUORESCENT BIOSENSOR FOR SIMULTANEOUS DETECTION OF HYDROGEN PEROXIDE AND PHOSPHATIDYL INOSITOL-3,4,5-TRIPHOSPHATE, NUCLEINIC ACID CODING SUCH PROTEIN, EXPRESSION CASSETTE AND EUCARIOTIC HOST CELL

摘要

FIELD: biotechnologies.SUBSTANCE: proposed chimeric protein with SEQ ID NO:02 is fluorescent biosensor, built on the basis of HyPer protein and mutant of PH-domain of Btk tyrosine kinase.EFFECT: proposed inventions allow performing simultaneous monitoring of product of hydrogen peroxide and phosphatidyl inositol-3,4,5-triphosphate in a living cell.4 cl, 4 dwg, 3 ex