| 摘要 |
An image processing program (GP) attains (S10) time lapse images, extracts (S20) a cell aggregation from each of the obtained images, and calculates (S50), for the entire region of the cell aggregation, the spatial distribution in pixel values and temporal change in pixel values of a small region in the extracted cell aggregation. The program determines (S70) the state of change of the cell aggregation toward becoming multi-layered, on the basis of the calculated spatial distribution and time lapse change of pixel values, and outputs (S80) the results of the determination. The image processing program (GP) is configured so as to determine and output, from time lapse images, the state of change of the cell aggregation toward becoming multi-layered.
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