USE OF ALPHA-AMYLASE FROM ASPERGILLUS CLAVATUS FOR SACCHARIFICATION
摘要
A fungal alpha-amylase is provided from Aspergillus clavatus (AcAmy1). AcAmy1 has an optimal pH of 4.5 and is operable at 30-75°C, allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AcAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1 + DP2) compared to the products of saccharification catalyzed by an alpha-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
申请公布号
CA2870830(A1)
申请公布日期
2013.11.14
申请号
CA20132870830
申请日期
2013.05.06
申请人
DANISCO US INC.
发明人
CLARKSON, KATHLEEN A.;GE, JING;HUA, LING;HUITINK, JACQUELYN A.;SCHEFFERS, MARTIJN SILVAN;TANG, ZHONGMEI;VAN BRUSSEL-ZWIJNEN, MARCO;VROEMEN, CASPER;ZHANG, BO;TEUNISSEN, PAULA JOHANNA MARIA