UROKINASE-TYPE PLASMINOGEN ACTIVATOR TRANSGENIC MOUSE

摘要

PROBLEM TO BE SOLVED: To provide a hepatopathy mouse with a high degree of disorder to original hepatocytes of the mouse while having a uPA gene in a heterozygous form, and a method for efficiently producing the mouse.SOLUTION: A method for producing a hepatopathy mouse with a uPA gene in a heterozygous form, includes the steps of: (i) transforming a mouse ES cell with a DNA fragment that contains a liver-specific promoter/enhancer and urokinase-type plasminogen activator-encoding cDNA that is operably linked under the control of the liver-specific promoter/enhancer; (ii) injecting the transformed mouse ES cell obtained in the step (i) into a host embryo; (iii) transplanting the host embryo obtained in the step (ii) and injected with the ES cell, into the uterus of a surrogate mother mouse to obtain a chimeric mouse; and (iv) mating the chimeric mouse obtained in the step (iii) to obtain a transgenic mouse in which the DNA fragment is introduced in the heterozygous form.