PROCESS FOR RENATURATION OF POLYPEPTIDES

摘要

The invention relates to method of refolding of proteins from a solution containing the protein in predominantly misfolded, aggregated form. The method involves denaturation and reduction of the protein of interest. The denatured and reduced preparation is subjected to removal of reducing agent in denaturing condition and at low pH to prevent the misfolding of the protein. The protein preparation is subjected to refolding followed by removal of the refolding buffer components.