MINIUIB MINITRANSPOSON AND DERIVED USES

摘要

The invention relates to a minitransposon comprising left and right inverted repeats of ISPst9, and to the use thereof for transferring a nucleic acid or for random mutagenesis. In addition, the invention relates to a method for the transposition of a nucleic acid from a donor bacterium to a recipient bacterium, comprising introducing the nucleic acid to be transposed between the inverted repeats of the minitransposon of the invention, introducing the minitransposon into the donor bacterium and conjugating the donor and recipient bacteria.