| 摘要 |
PROBLEM TO BE SOLVED: To provide novel regenerative medical treatment inducing functional tissue regeneration by mobilizing a large amount of stem cells to damaged sites.SOLUTION: This invention is based on the following: (1) a large amount of bone marrow-derived cells are mobilized in transplanted skin; (2) the mobilized bone marrow-derived cells are differentiated into any of dermal fibroblasts, adipocytes, muscle cells, vascular endothelial cells and epidermal keratinocytes in a transplanted skin graft, and in the mobilized bone marrow-derived cells, bone marrow-derived mesenchymal stem cells are contained; (3) what mobilize the bone marrow-derived mesenchymal stem cells to the graft from the peripheral blood are HMGB1, HMGB2 and HMGB3 released from necrotic tissue of the transplanted skin; (4) purified HMGB1, HMGB2 and HMGB3 promote migration of mesenchymal stem cells isolated from the bone marrow and cultured; (5) active substances including HMGB1 can be simply purified from an extract of a large number of organs including skin, brain and heart; (6) active substances allowing bone marrow mesenchymal stem cells to migrate can be simply extracted from cultured cells; and (7) a heparin column-purified fraction of a skin extract mobilizes a large amount of bone marrow-derived cells during brain damage. |
