发明名称 |
PLASMID, TRANSFORMANT, AND METHOD FOR PRODUCTION OF 3-CARBOXYMUCONOLACTONE |
摘要 |
There is provided a process for industrial production of simple 3-carboxy-cis,cis-muconic acid and/or 3-carboxymuconolactone from low molecular mixtures derived from plant components such as vanillin, vanillic acid and protocatechuic acid, via a multistage enzyme reaction. A recombinant plasmid containing a vanillate demethylase gene (vanAB genes), benzaldehyde dehydrogenase gene (ligV gene) and protocatechuate 3,4-dioxygenase gene (pcaHG genes); transformants incorporating the plasmid; and a process for production of 3-carboxy-cis,cis-muconic acid and/or 3-carboxymuconolactone characterized by culturing the transformants in the presence of vanillin, vanillic acid, protocatechuic acid or a mixture of two or more thereof. |
申请公布号 |
EP2048231(B1) |
申请公布日期 |
2013.11.06 |
申请号 |
EP20070792616 |
申请日期 |
2007.08.10 |
申请人 |
FORESTRY AND FOREST PRODUCTS RESEARCH INSTITUTE |
发明人 |
MASE, KOHEI;SHIMO, TOSHIHISA;OHARA, NAOKI;KATAYAMA, YOSHIHIRO;SHIGEHARA, KIYOTAKA;MASAI, EIJI;FUKUDA, MASAO;OHARA, SEIJI;NAKAMURA, MASAYA;OTSUKA, YUICHIRO |
分类号 |
C12N15/00;C12N1/21;C12N15/09;C12P7/26;C12P7/40 |
主分类号 |
C12N15/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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