发明名称 |
Chorismate mutase gene from the potato cyst nematode Globodera rostochiensis |
摘要 |
The nucleotide sequence of a 992 bp region of cDNA and the nucleotide sequence of a 1973 bp (or a 1913 bp) of genomic DNA of the Gr-cm-1 gene were determined for G. rostochiensis. PCR primers and probes specific for G. rostochiensis and G. pallida were generated. PCR assays, including a real-time TaqMan PCR were used to identify G. rostochiensis and G. pallida and to differentiate G. rostochiensis from G. pallida. Transgenic hairy roots expressing Gr-cm-1 dsRNA were generated. There was a 52% reduction in the average number of females per root in the Gr-cm-1 dsRNA transgenic lines when compared with the infected control lines.
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申请公布号 |
US8575427(B2) |
申请公布日期 |
2013.11.05 |
申请号 |
US20090460813 |
申请日期 |
2009.07.24 |
申请人 |
WANG XIAOHONG;LU SHUNWEN;THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF AGRICULTURE |
发明人 |
WANG XIAOHONG;LU SHUNWEN |
分类号 |
A01H1/02;C12N15/113;C12N15/64;C12N15/74;C12P19/34;C12P21/06 |
主分类号 |
A01H1/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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