| 摘要 |
The invention relates to chemistry and biotechnology, in particular to the synthesis of a new coordinative compound of cobalt(II) and to a process for cultivation of microalga Porphyridium cruentum with the use thereof.According to the invention, a coordinative compound - tetraisothiocyanatocobaltate of bis(nicotinoylhydrazone)-2,6-diformil-4-methylphenol(methanol)(aqua)cobalt(II) is claimed.Also, a process for cultivation of microalga Porphyridium cruentum is claimed, which consists in that microalga is cultivated on a nutrient medium containing, g/L: NaNO3 - 5.0; NaCl - 7.0; KCl - 7.5; MgSO4·7H2O - 1.8; Ca(NO3)2·4H2O - 0.15; KBr - 0.05; KI - 0.05; K2HPO4 - 0.2; FeCl3·6H2O - 0.00027; ZnSO4·5H2O - 0.00002; CuSO4·5H2O - 0.00005; MnSO4·5H2O - 0.0003; H3BO3 - 0.0006; MoO3 - 0.00002; NaVO3 - 0.00005; the compound tetraisothiocyanatocobaltate of bis(nicotinoylhydrazone)-2,6-diformil-4-methylphenol(methanol)(aqua)cobalt(II) - 0.021…0.023 g/L and distilled water up to 1L, having the pH 6.8…7.2; at the temperature of 23…25°C, the lighting of 2000…3000 lx/cm2, with slow intermittent agitation.The result consists in increasing the antioxidant activity of the ethylic extract obtained from microalga biomass. |
