发明名称 |
PRODUCTION OF NUCLEIC ACID |
摘要 |
A method for the production of nucleic acid encoding a target protein. The method comprises (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain no more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.
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申请公布号 |
US2013288925(A1) |
申请公布日期 |
2013.10.31 |
申请号 |
US201313921989 |
申请日期 |
2013.06.19 |
申请人 |
JANULAITIS ARVYDAS;SKIRGAILA REMIGIJUS;SIKSNIENE DANGIRA |
发明人 |
JANULAITIS ARVYDAS;SKIRGAILA REMIGIJUS;SIKSNIENE DANGIRA |
分类号 |
C12P19/34 |
主分类号 |
C12P19/34 |
代理机构 |
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地址 |
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