发明名称 |
PROCESO DE PURIFICACION Y ESTABILIZACION DE LA ENZIMA GLUCONATO DESHIDROGENASA (GADH, EC 1.1.99.3); ENZIMA GLUCONATO DESHIDROGENASA (GADH, EC 1.1.99.3); Y USO DE LA ENZIMA GLUCONATO DESHIDROGENASA (GADH, EC 1.1.99.3). |
摘要 |
The invention relates to a method for the purification and stabilisation of enzyme gluconate dehydrogenase (GADH, EC 1.1.99.3), optionally recombinant, from multiple microorganisms, for example: Pseudomonas aeruginosa, Pseudomonas fluorescens, Gluconobacter oxydans, Gluconobacter industrius, Serratia marcescens, Klebsiella pneumoniae and Escherichia coli, and to the use thereof as a biological recognition element in biosensors for detecting gluconic acid in samples of interest. The invention also relates to a biocatalytic biosensor with free electrochemical transduction of interferences owing to the high selectivity of the enzyme obtained using the aforementioned method and stabilised with optimised chemical agents. |
申请公布号 |
ES2387661(B1) |
申请公布日期 |
2013.10.31 |
申请号 |
ES20100031819 |
申请日期 |
2010.12.10 |
申请人 |
BIOLAN MICROBIOSENSORES S.L. |
发明人 |
ALONSO RODRIGUEZ, PABLO;QUIROS FERNANDEZ, LUIS MANUEL;CASTANON DE LA TORRE, SONIA;CRESPO SUSPERREGUI, AINARA;MAZA DEL RIO, SONIA |
分类号 |
C12N9/02;C12H1/15;C12N9/04 |
主分类号 |
C12N9/02 |
代理机构 |
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