Reagents and tests designed for screening and follow-up of type 2 diabetes mellitus

摘要

PURPOSE: A reagent for diagnosis and follow-up study on type 2 diabetes and a test method including a measurement of IL-1 beta are provided to accurately diagnose type 2 diabetes with convenience and comfortableness. CONSTITUTION: A novel method for diagnosing type 2 diabetes comprises the steps of: inducing macrophage from monocytes collected from blood of a person who is suspected to have diabetes and culturing the macrophage; measuring the concentration of active IL-1 beta secreted from the cultured macrophage; and determining type 2 diabetes when the concentration of active IL-1 beta is over a predetermined level. A method for determining treatment effects on type 2 diabetes comprises the steps of: inducing macrophage from monocytes collected from a patient's blood with a type 2 diabetes during treatment and culturing the macrophage; measuring the concentration of active IL-1 beta secreted from the cultured macrophage; and determining that a treatment has an effect on the patient when the concentration of active IL-1 beta is less than a predetermined level. [Reference numerals] (A) Patient with type 2 diabete before meformin treatment; (B) Patient with type 2 diabete after meformin treatment; (H) Ordinary person