METHODS FOR PREPARING TRANSFORMED E.COLI FOR EXPRESSION OF PHOSPHOENOLPYRUVATE CARBOXYLASE

摘要

PURPOSE: A method for preparing transformed E. coli for expressing phosphoenolpyruvate carboxylase (PEPC) is provided to obtain a large amount of PEPC, to improve the activity of PEPC, and to produce industrially useful materials. CONSTITUTION: A method for preparing transformed E. coli for expressing PEPC comprises the steps of: acquiring a nucleotide sequence of sequence number 1 of PEPC of Glaciecola sp. by codon optimization for expressing the nucleotide sequence in E. coli; inserting the codon-optimized nucleotide sequence into an expression vector and preparing a recombinant vector; and transducing the recombinant vector to E. coli and preparing transformed E. coli.