摘要 |
PURPOSE: A spectrophotometric quantitation of an anionic surfactant is provided to reduce analysis time to 1/3, the dose of a solvent to 1/2, and the size of an analysis container to 1/2, thereby largely reducing analysis time and cost. CONSTITUTION: A spectrophotometric quantitation of an anionic surfactant comprises the steps of: adding a pretreatment reagent, a methylene blue (MB) solution, and a mixture (MIBK-EDC) of methyl isobutyl ketone and 1,2-dichloroethane to seawater or an environmental water sample and mixing and incubating the primary mixture; removing an aqueous layer from the incubated mixture, adding primary washing water, and mixing and incubating the secondary mixture; adding secondary washing water and mixing and incubating the tertiary mixture; removing an aqueous layer from the tertiary mixture and filtering the remaining liquid; and measuring absorbance of the remaining liquid. [Reference numerals] (AA) Sample; (BB) Pretreatment reagent; (CC) Extraction (Mixing/Incubation); (DD,GG,JJ) Remove an aqueous layer; (EE) Primary washing water; (FF,II) Washing(Mixing/Incubation); (HH) Secondary washing water; (KK) 1ps filter paper; (LL) Filter; (MM) Measure absorbance |