METHOD OF PRODUCING A LIPOLYTIC ENZYME

摘要

Patent No. 595833 Disclosed is a method of producing a lipolytic enzyme comprising the steps of: (i) providing a transformed or transfected Trichoderma reesei cell comprising at least one heterologous nucleotide sequence encoding a lipolytic enzyme; (ii) culturing the cell at a of pH 4 to a pH of 5.5 under conditions to allow for expression of said heterologous nucleotide sequence(s) encoding said lipolytic enzyme; and (iii) isolating, purifying or concentrating the enzyme in a medium at a pH of 5.5 to a pH of 6.5.