SPECIFIC BIOMARKER FOR IDENTIFICATION OF EXPOSURE TO PROPIONALDEHYDE AND THE METHOD OF IDENTIFICATION USING THESAME

摘要

PURPOSE: A biomarker for identifying propionaldehyde-specific exposure and an identification method using the same are provided to monitor propionaldehyde and to determine risks using a response gene selected by a DNA microarray chip. CONSTITUTION: A DNA microarray chip for identifying propionaldehyde-specific exposure contains nucleic acid sequences of one or more genes selected among angiotensinogen (AGT) gene (Genbank accession number NM_0000299), lysophosphatidic acid receptor 1 (LPAR1) gene (Genbank accession number NM_057159), secreted and transmembrane 1 (SECTM1, Genbank accession number NM_003004), tumor necrosis factor (ligand) superfamily, member 10 (TNFSF10) gene (Genbank accession number NM_003810), and heme oxygenase 1 (HMOX1) gene (Genbank accession number NM_002133) or a complementary strand thereof. A method for identifying propionaldehyde exposure comprises the steps of: isolating RNAs from somatic cells of an experimental group and a normal control group; synthesizing cDNAs from the RNAs; labeling the cDNAs with different fluorescent materials; hybridizing the cDNAs onto a DNA microarray chip; analyzing the DNA microarray chip; and comparing the expression levels of genes of the control group and the experimental group, integrated on the DNA microarray chip.