发明名称 |
STEM CELL AGGREGATE SUSPENSION COMPOSITIONS AND METHODS FOR DIFFERENTIATION THEREOF |
摘要 |
Disclosed is a bioreactor comprising primate pluripotent stem cell-derived cell aggregates in suspension, wherein the primate pluripotent stem cell-derived cell aggregates in suspension are generated by a method comprising: a. providing a culture of undifferentiated adherent primate pluripotent 5 stem cells; b. disassociating the adherent primate pluripotent stem cell culture of step (a) into a primate pluripotent stem single cell suspension; c. seeding the primate pluripotent stem cell single cell suspension in a defined culture medium that supports undifferentiated growth of the primate pluripotent stem cells, thereby forming a primate pluripotent stem cell single cell suspension culture; d. agitating the primate pluripotent stem single cell suspension culture at low shear, wherein the primate pluripotent stem single cells in the suspension form substantially uniform primate pluripotent stem cell aggregates; and e. contacting the primate pluripotent stem cell aggregates in suspension with a differentiating culture condition wherein primate pluripotent stem cell aggregates in suspension from primate pluripotent stem derived cell aggregates in suspension, thereby generating primate pluripotent stem derived cell aggregates in suspension.
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申请公布号 |
NZ600082(A) |
申请公布日期 |
2013.10.25 |
申请号 |
NZ20120600082 |
申请日期 |
2012.05.18 |
申请人 |
VIACYTE, INC. |
发明人 |
GREEN, CHAD;YU, XIAOJIE;BANG, ANNE;BRANDON, EUGENE P |
分类号 |
C12N5/00;C12N5/02 |
主分类号 |
C12N5/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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