| 摘要 |
595982 Disclosed is a method for isolating a target bioentity from a biological sample, which comprises the steps of: (a) contacting said biological sample with a magnetically-labelled ligand having selective binding affinity for the target bioentity, or for a determinant on the target bioentity, to form a target bioentity/labelled ligand complex in said biological sample; (b) locating a separation module having a defined fluid flow path in the magnetic field of a magnetic module, said magnetic module comprising an array of at least two magnets in which adjacent magnets in the array are aligned with opposing polarity; (c) passing said biological sample through the separation module to subject the biological sample to the magnetic field while the sample is passing through the defined fluid flow path to magnetically capture the target bioentity/labelled ligand complex by arresting or hindering movement of said complex within the defined fluid flow path; (d) removing the separation module from the magnetic field; and (e) recovering said target bioentity/labelled ligand complex from the fluid flow path, wherein recovery of the target bioentity/labelled ligand complex includes the step of flushing said complex from the defined fluid flow path by passing one or more aliquots of wash solution through the flow path, and wherein said flushing step comprises back-flushing through the defined fluid flow path. Further disclosed is an apparatus for use in isolating a target bioentity from a biological sample which comprises: (i) a separation module having a defined fluid flow path; (ii) a magnetic module having a magnetic field, said magnetic module comprising an array of at least two magnets in which adjacent magnets in the array are aligned with opposing magnetic polarity; wherein the separation module has a first position in which the fluid flow path is located within the magnetic field of the magnetic module and a second position in which the fluid flow path is removed from said magnetic field; and (iii) a controller comprising means for passing a biological sample, and optionally a wash solution, through the defined fluid flow path provided by the separation module when the separation module is located within the magnetic field whereby the biological sample is subjected to the magnetic field of the magnetic module while the sample is passing through the defined fluid flow path, and for flushing the defined fluid flow path by back-flushing wash solution through the defined fluid flow path when the separation module is removed from the magnetic field.
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